Figure 2.

Tissue-Specific Expression of the LjPLP-I, LjPLP-II, and LjPLP-IV Genes.

(A) Fragments (400-bp), encompassing either the UAG₂₂₂ stop codon or the CAG₂₂₂ triplet-containing regions of LjPLP-I and LjPLP-II mRNAs, respectively, were amplified using RT-PCR. The 400- and 200-bp fragments observed in the samples digested with Eco57I correspond to the LjPLP-I and LjPLP-II transcripts, respectively.

(B) Tissue-specific expression of the LjPLP-IV transcripts. Ten micrograms of total RNA was hybridized with radiolabeled, strand-specific, RNA probes complementary to the presumed sense or antisense transcripts of the LjPLP-IV gene. The positions of 28S and 18S rRNAs are indicated.