Figure 5.

The Internal Bidirectional Promoter of the LjPLP-IV Gene Directs the Expression of the β-Glucuronidase Reporter Gene to the Central Infected Zone of L. corniculatus Nodules.

(A) Nucleotide sequence of the intron-localized bidirectional promoter of the LjPLP-IV gene. The directions of transcription of the LjPLP-IV antisense transcripts and the LjNOD16 transcript are indicated by arrows. The positions and presumed orientations of TATA box–like sequences are indicated by arrows and nodulin gene conserved promoter motifs are indicated by boxed sequences.

(B) Histochemical localization of GUS activity in nodule hand sections derived from transgenic L. corniculatus plants harboring the promoter–GUS fusions. VB, nodule vascular bundle (for further details see text).