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. 2026 Jun 26;17(1):791. doi: 10.1038/s41419-026-09054-9

Fig. 1. H2AJ expression is downregulated in LUAD.

Fig. 1

A Comparison of H2AJ expression in normal and tumor tissues of patients with LUAD (P = 1.2 × 10−10 for GSE31210 and 1.4 × 10−14 for GSE10072, t-test). B Overall survival rates of patients with LUAD classified by H2AJ expression (P = 6.9 × 10−3 for GSE50081 (n = 127) and 7.3 × 10−4 for GSE31210 (n = 204), log-rank test). C Schematic diagram of the two methylation sites in H2AJ promoter. D Comparison of DNA methylation patterns in normal and tumor tissues of patients with LUAD (P = 1.6 × 10−5 for cg26127836 and 2.4 × 10−5 for cg07112131, t-test). E Correlation between DNA methylation patterns and H2AJ expression in patients with LUAD (P = 6.1 × 10−3 for cg26127836 and 2.3 × 10−3 for cg07112131, Spearman’s rank correlation coefficient). F DNA methylation values of two methylated probes in A549 and A427 cells treated with 5-aza-deoxycytidine (5-Aza-dC) for 24 h. G H2AJ mRNA expression in A549 and A427 cells treated with 5-Aza-dC for 24 h. H2AJ mRNA expression levels were normalized to those of GAPDH. H Immunoblot validation of H2A.J in A549 and A427 cells treated with 5-Aza-dC for 24 h. Error bars show the mean ± standard error of the mean (SEM) of three biological replicates; **P < 0.01, ***P < 0.001 vs. control.