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. 2026 Jun 26;17(1):791. doi: 10.1038/s41419-026-09054-9

Fig. 5. TMEM158 is a target gene of H2A.J.

Fig. 5

A Results of Venn analysis between ChIP-Seq and RNA-Seq data using H2AJ overexpressing A549 cells. B Heat map of putative target gene expression changes. C Integrated analysis of TMEM158 gene. The shaded blue boxes represent the promoter sites to which H2A.J binds in the ChIP-seq analysis. D Validation of H2A.J binding to promoter regions of TMEM158 and unchanged gene (GAPDH) using a ChIP-PCR assay. E RT-qPCR and immunoblot validation of TMEM158 in H2AJ overexpressing A549 cells. F H3K27me3 at TMEM158 promoter in H2AJ overexpression condition. G Results of Invasion assays with H2AJ, TMEM158 overexpression alone or in combination. H The immunoprecipitation analysis for interaction between H2A.J and EZH2 after ectopic H2AJ overexpression in A549 cell. I Binding of EZH2 at TMEM158 promoter in H2AJ overexpression condition. J, K H3K27me3 at TMEM158 promoter (J) and RT-qPCR validation of TMEM158 expression (K) in H2AJ overexpression alone or with EZH2 knockdown. L, M Reporter construct with TMEM158 promoter (L) and luciferase assay results using the reporter system with promoters in HEK293 cells (M). N Repression of transcriptional machinery on TMEM158 promoter by H2AJ overexpression. Error bars show the mean ± standard error of the mean (SEM) of 3 biological replicates, *P < 0.05, **P < 0.01, ***P < 0.001 vs control; ns not significant.