FIG. 3.

eIF4E^Ser251Ala mutant flies have reduced growth. Rescue of the eIF4E^589/11 allele with the P{eIF4E^Ser251Ala} transgene results in adult flies with small body size and aberrant wing growth (A), while flies carrying the P{eIF4E^Ser251Asp} transgene in the background of the eIF4E^589/11 allele are similar to controls (B). Males (top row) and females (bottom row) are shown. The control flies, which were grown under conditions identical to those for the transgenic mutants, are siblings of eIF4E^Ser251Ala (P{eIF4E^Ser251Ala} eIF4E^589/11/TM3 Sb) (A) and eIF4E^Ser251Asp (P{eIF4E^Ser251Asp} eIF4E^589/11/TM3 Sb) (B) flies. Adult compound eyes of control (C), eIF4E^Ser251Ala (D), and eIF4E^Ser251Asp (E) male flies in the background of the eIF4E^589/11 allele are also shown. Note the reduction in size of eIF4E^Ser251Ala mutant eyes compared to those of control and eIF4E^Ser251Asp flies. The control shown is from a male of genotype P{eIF4E^Ser251Ala} eIF4E^589/11/TM3 Sb, which was grown under conditions identical to those for the eIF4E^Ser251Ala and eIF4E^Ser251Asp mutants. Similar results were obtained with female fly eyes (data not shown). Magnification for all electron micrographs, ×160. Bar = 100 μm. (F) Average masses of eIF4E^Ser251Ala and eIF4E^Ser251Asp mutants compared to those of their respective control siblings (n = 60). (G and H) Relative areas of individual ommatidia (G) and average numbers of ommatidia per compound eye (H) of males and females (n = 5 individuals for each genotype). C₁, control sibling of eIF4E^Ser251Ala (P{eIF4E^Ser251Ala} eIF4E^589/11/TM3 Sb) mutant; C₂, control sibling of eIF4E^Ser251Asp (P{eIF4E^Ser251Asp} eIF4E^589/11/TM3 Sb) mutant; A, P{eIF4E^Ser251Ala} eIF4E^589/11 mutant; D, P{eIF4E^Ser251Asp} eIF4E^589/11 mutant. ∗, significant P values, as calculated using a one-tailed Student t test comparing the average masses of male and female mutants to those of their control siblings. Data represent means ± standard deviations.