FIG. 5.

Quantitative analysis of IFN-γ poly(A) in nuclei of NK cells. NK 92 cells were rested in the absence of IL-2 and IL-15 for 12 h and then stimulated with IL-12 (10 U/ml) for the times indicated. Nuclear RNA was isolated and reverse transcription-PCR was performed with random hexamers and oligo(dT) as primers for reverse transcription. Quantitative real-time PCR was performed on the resulting cDNAs with an IFN-γ-specific Taqman probe and primers specific for the 3′ UTR of the IFN-γ gene. The amount of DNA synthesized from the random hexamer (RH)-generated cDNA (open squares) was compared to the amount produced from the oligo(dT)-generated cDNA (solid triangles). Data for each time point represent the mean values of three separate experiments ± two standard deviations.