FIG. 1.

Identification of a Drosophila geminin homologue. (A) Homology between human (Homo sapiens), Xenopus (Xenopus laevis), and Drosophila (Drosophila melanogaster) geminin proteins. Xenopus has two cDNAs encoding geminin H and L. Only geminin H is shown. (B) Association between geminin and Dup. The geminin and Cdt1/Dup proteins were immunoprecipitated from SD2 cell lysates with antigeminin, preimmune serum, and anti-Cdt1/Dup antibodies. Western blotting was performed with anti-Cdt1/Dup (top) and antigeminin (bottom) antibodies. The molecular mass standards are indicated on the left (in kilodaltons). Cdt1/Dup migrates at about 98 kDa, and the faster migrating band is likely a proteolytic fragment.