Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Mar;22(6):1868–1880. doi: 10.1128/MCB.22.6.1868-1880.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology.

PMC Copyright notice

FIG. 4. — Morphological analysis of silencing geminin or Cdt1/Dup and cosilencing geminin and Cdt1/Dup. The cells were incubated with dsRNAs. At various times, the cells were fixed and the nuclei were stained with DAPI. The nuclear stainings were examined under the same magnification. (A to C) The control (Neo) knockout. (D to F) The geminin knockout. Note the induction of giant nuclei containing densely and diffusely stained regions in geminin-deficient cells. (G to I) The Cdt1/Dup knockout. Note the accumulation of unusual, small cells after the silencing of Cdt1/Dup. (J to L) The geminin and Cdt1/Dup double knockout. The morphological suppression of geminin deficiency by the cosilencing of Cdt1/Dup is shown. (M) Western blot analysis of knockout samples for control (Neo), geminin (Gem), Cdt1/Dup (DUP), and geminin plus Cdt1/Dup (GEM + DUP) knockouts, as shown in panels A to L and in Fig. 3.

FIG. 4. — Morphological analysis of silencing geminin or Cdt1/Dup and cosilencing geminin and Cdt1/Dup. The cells were incubated with dsRNAs. At various times, the cells were fixed and the nuclei were stained with DAPI. The nuclear stainings were examined under the same magnification. (A to C) The control (Neo) knockout. (D to F) The geminin knockout. Note the induction of giant nuclei containing densely and diffusely stained regions in geminin-deficient cells. (G to I) The Cdt1/Dup knockout. Note the accumulation of unusual, small cells after the silencing of Cdt1/Dup. (J to L) The geminin and Cdt1/Dup double knockout. The morphological suppression of geminin deficiency by the cosilencing of Cdt1/Dup is shown. (M) Western blot analysis of knockout samples for control (Neo), geminin (Gem), Cdt1/Dup (DUP), and geminin plus Cdt1/Dup (GEM + DUP) knockouts, as shown in panels A to L and in Fig. 3.