FIG. 6.

Drosophila Chk1/Grapes silencing suppresses geminin deficiency-induced polyploidy and giant nucleus formation. (A and B) Flow cytometry analysis. Control (Neo) is indicated by a thin line; geminin silencing is indicated by a thick line; Chk1/Grapes and geminin double knockout is indicated by a dotted thin line. (C to J) Nuclear morphological analysis of control (Neo) knockout (C and D), geminin knockout (E and F), Chk1/Grapes knockout (G and H), and geminin and Chk1/Grapes double knockout (I and J). The same magnification was used for all panels. (K) Western blot of geminin (top) and Northern blot of Chk1/Grapes (bottom) in the knockout samples. Abbeviations are the same as those for Fig. 4M.