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. 2002 Mar;22(6):1778–1791. doi: 10.1128/MCB.22.6.1778-1791.2002

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Copyright © 2002, American Society for Microbiology.

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FIG. 2. — Identification of SAP97 as an mLin-2/CASK L27N domain binding partner. (A) Lysate (lane 1) and proteins immunoprecipitated (IP) with preimmune serum (lane 2) or with an anti-Myc antibody from MDCK cell lysates stably expressing mLin-2/CASK (lane 3) were separated by SDS-PAGE and transferred to nitrocellulose. The membrane was then probed with an anti-SAP97N terminus antibody (A), stripped and probed with a ³²P-labeled GST-mLin-2/CASK L27N protein probe (B), or stripped and probed with an anti-Myc antibody (C).