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. 2002 Mar;22(6):1778–1791. doi: 10.1128/MCB.22.6.1778-1791.2002

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Copyright © 2002, American Society for Microbiology.

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FIG. 3. — Direct interaction between L27N domain of mLin-2/CASK and SAP97. (A) Domain structure of mLin-2/CASK. Numbers, amino acid positions of various domain limits. (B) HEK 293T cells were transiently transfected with Myc-SAP97. Myc-SAP97 from Triton X-100-extracted cell lysates was precipitated with GST (lane 2), GST-mLin-2/CASK (L27N) (lane 3), GST-mLin-2/CASK (GK) (lane 4), GST-mLin-2/CASK (SH3-GK) (lane 5), or GST-mLin-2/CASK (PDZ-SH3-GK) (lane 6). The precipitated mLin-2/CASK proteins were detected with an anti-Myc antibody (top). Equivalent amounts of GST fusion proteins were revealed with Ponceau red stain (bottom). Molecular masses in kilodaltons are shown to the left. FL, full length.