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. 2002 Mar;22(6):1778–1791. doi: 10.1128/MCB.22.6.1778-1791.2002

FIG. 5.

FIG. 5.

SAP97, mLin-2/CASK, and mLin-7 form a stable complex in a HEK 293 and MDCK cells. (A) Collected lysates from MDCK cells were precipitated with preimmune serum, an anti-SAP97 antibody, an anti-mLin-2/CASK antibody, and an anti-mLin-7 antibody. Precipitated proteins were separated on an SDS-10% PAGE gel and transferred to a nitrocellulose membrane. The top portion of the membrane was immunoblotted with an anti-mLin-2/CASK antibody, and the bottom portion of the membrane was immunoblotted with an anti-mLin-7 antibody. The top portion of the membrane was then stripped and reprobed with radiolabeled 32P-GST-L27N to detect SAP97. IP, immunoprecipitation. (B and C) The same procedures were performed with lysate from MDCK cells stably expressing X11α (B) and HEK 293 cells (C).