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. 2002 Mar;22(6):1778–1791. doi: 10.1128/MCB.22.6.1778-1791.2002

FIG. 9.

FIG. 9.

Evolutionary conservation of the interaction between the SAP97 N terminus and the mLin-2 L27N domain in Drosophila. (A) Amino acid sequence of full-length CPD protein (B) Schematic of Drosophila chromosomal region containing the coding sequences for CPD and Dlg and the primary structure of the CPD-Dlg splice variant. (C) Sequence alignment of the first 65 aa residues of SAP97, CPD, and C. elegans DLG-1 proteins. (D) Interaction between Myc-Camguk(1-500) and GST-CPD. Lysates from HEK 293 cells transiently expressing Myc-Camguk(1-500) were incubated with GST, GST-CPD(27-104), and GST-CPD(1-65) proteins. (E) Interaction between Myc-mLin-2/CASK and GST-CPD. Lysates from MDCK cells stably expressing Myc-mLin-2/CASK proteins were precipitated with GST, GST-CPD(25-104), GST-CPD(1-65), and GST-SAP97(1-78). (F) Interaction between GST-Camguk L27N domain and the full-length Myc-CPD protein. Lysates from HEK 293 cells transiently expressing full-length Myc-CPD were incubated with GST and the GST-Camguk L27N domain.