Fig. 6.

FCG model validation via juvenile somatic gonads weight measures. A-C In gonadal males, XYM mice had heavier testis, seminal vesicle and bulbocavernosus/levator ani (BCLA) weights than XXM mice. Early MIA decrease testis weight in XYM mice, but increased BCLA weight in XXM relative to saline-treated controls (all ps < 0.05). D-E In gonadal females, regardless of MIA treatment, XYF mice have lower uterine horn and ovaries weight than XXF mice (all ps < 0.05). Early MIA significantly decreased ovaries weight whereas late MIA decreased uterine horn weight in XXF mice, relative to saline-treated controls (all ps < 0.05). Overall, the mismatch XXM and XYF groups showed lower gonad weight than typical XYM and XXF mice. Although previous studies indicate comparable circulating gonadal hormones in the FCG mice with the same gonad type, these differences in gonadal weight may have implications for their reproduction success. Two-way ANOVA with Tukey’s post hoc tests, n = 11–12 per group. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. Graphs created in GraphPad Prism