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. 2002 Mar;22(6):1919–1925. doi: 10.1128/MCB.22.6.1919-1925.2002

FIG. 5.

FIG. 5.

Number of totipotent cells in ATF1−/− CREB−/− embryos and of pluripotent cells in ATF1+/− CREB−/− embryos is strongly reduced. (A) oct-4 expression in wild-type and ATF1+/− CREB−/− embryos at E6.0 and E7.5 detected by in situ hybridization. Pluripotent cells of the epiblast appear positive for Oct-4. In contrast to the wild type, ATF1+/− CREB−/− embryos develop a strongly reduced embryonic part with fewer epiblast cells or lack pluripotent cells completely. (B) Hoechst staining reveals that ATF1−/− CREB−/− embryos are comprised of fewer blastomeres than are their wild-type littermates.