FIG. 8.

Polyamide 3 inhibition of the TFIID-TFIIA-DNA complex analyzed by EMSA. (A) Radiolabeled D+15 DNA was incubated with either no polyamide (lanes −) or with 25, 50, and 200 nM polyamide 3 or 200 nM polyamide 1 for 15 min prior to the addition of TFIID plus TFIIA, as indicated. After a subsequent 20-min incubation, the samples were subjected to electrophoresis. Only the region of the gel containing the TFIID-TFIIA-DNA (DA-DNA) complex is shown. (B) Radiolabeled U−15 DNA was incubated with either no polyamide or with 50, 100, and 200 nM polyamide 3 or 200 nM polyamide 2 for 15 min prior to the addition of TFIID plus TFIIA, as for panel A. (C) Phosphorimage quantitation of the extent of DA-DNA complex formation with increasing concentrations of polyamide 3. Data are normalized to the phosphorimage units in the DA-DNA complex in the absence of polyamide.