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. 2002 Mar;22(6):1903–1918. doi: 10.1128/MCB.22.6.1903-1918.2002

FIG. 8.

FIG. 8.

Fesact requires its kinase activity, a functional SH2 domain, and Tyr 713 for biological activity. (A) U937 cells were infected with MigR1, MigR1 Fesact, MigR1 FesactR483L, MigR1 FesactK590R, and MigR1 FesactY713F viruses. Five days after infection, cells were stained with anti-CD11b antibody and analyzed by flow cytometry. (B) Portions of the U937 cells infected with MigR1 (lane 1), MigR1 Fesact (lane 2), MigR1 FesactR483L (lane 3), MigR1 FesactK590R (lane 4), and MigR1 FesactY713F (lane 5) viruses described in the panel A legend, above, were lysed 5 days after infection. The cell lysates were analyzed by SDS-PAGE in 8.5% gels followed by immunoblotting (WB) with the indicated antibodies either directly or after immunoprecipitation (IP) with anti-Fes antibodies. Top and upper-middle panels, immunoblotting of total cellular proteins with antiphosphotyrosine or anti-Fes antibodies, respectively. Lower-middle and bottom panels, immunoprecipitates of Fes were immunoblotted with anti-phosphotyrosine or anti-Fes antibodies, respectively. Numbers at the right indicate the positions of MW markers. Arrows indicate the positions of Fes proteins.