FIG. 1.

Affinity purification of the yeast SWI/SNF complex. (A) Diagram of the SWI/SNF purification scheme. Double affinity-purified yeast SWI/SNF complex was used in photo-cross-linking experiments. (B) The purified complex was separated on an SDS-4 to 15% gradient polyacrylamide gel and found to be largely free of contaminating proteins as visualized by silver staining. Known SWI/SNF subunits are indicated to the right of the panel, and unknown proteins are labeled according to their approximate molecular weight. E#1 and E#2 indicate two subsequent Flag peptide elutions from the Flag M2 agarose affinity gel.