FIG. 3.

Interaction of Snf5, Swi1, and Swi2/Snf2 in the context of intact SWI/SNF with Gcn4. (A) Photo-cross-linking reactions were carried out as in Fig. 2C, except that full-length Gcn4 (the GST tag was cleaved and removed) was conjugated with the cross-linking reagent and incubated with purified SWI/SNF. The asterisks indicate photo-cross-linked SWI/SNF subunits. (B) Reaction mixtures identical to those shown in panel A were separated on an SDS-5% polyacrylamide gel, instead of an SDS-4 to 15% gradient polyacrylamide gel. The asterisks indicate photo-cross-linked products.