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Identification of biotinylated SWI/SNF subunits after photo-cross-linking. Reaction mixtures identical to those shown in Fig. 3 were heat denatured in the presence of SDS, followed by incubation with streptavidin-agarose beads. The precipitated material was separated on an SDS-4 to 15% gradient polyacrylamide gel, transferred to PVDF, and immunoblotted with SWI/SNF-specific antisera.
