Figure 9.
Nuclear localization of p27S10A is maintained in lung tumors. (A) Lung tumor histology. Five-micron-thick slices of paraffin-embedded lungs tissues from wild-type, p27+/–, p27–/–, and p27S10A/S10A mice were stained with eosin and hematoxylin. Tumors were classified as bronchioalveolar adenomas and papillary adenomas. (B) p27 immunohistochemistry of lung tumors and normal surrounding tissue. Five-micron-thick slices of paraffin-embedded lung tissues from wild-type, p27+/–, and p27S10A/S10A mice were stained using the mouse monoclonal p27 antibody (clone 57; BD-Transduction Laboratories). (C) Western blot analysis of normal lung tissue and individual tumors form wild-type, p27+/–, and p27S10A/S10A mice. Forty micrograms of proteins were loaded per well. Membranes were probed successively with polyclonal antibodies to phospho-Ser10 p27, p27, PCNA, phospho-ERK (activated), and with a monoclonal anti-Grb2 antibody for protein loading.