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. 2006 Jan 1;20(1):87–100. doi: 10.1101/gad.357006

Figure 3.

Figure 3.

Premature phosphorylation of eIF2α blocks translation of genomic SV RNA. (A) Scheme of recombinant SV expressing luciferase from the genomic RNA. (B) Timing of eIF2α phosphorylation and translation of genomic and subgenomic 26S mRNA in PKR+/+ and PKR0/0 cells. Cells were infected with Toto-Luc1101 virus (MOI: 10 PFU/cell), and extracts were prepared at the times indicated. Luciferase activity was measured and used to quantitate translation from genomic SV RNA. Arrows indicate the time at which genomic RNA stopped translating. Translation from subgenomic mRNA was measured by Western blot of viral glycoprotein E1. (C) Effect of premature eIF2α phosphorylation on SV genomic RNA translation. PKR0/0 cells were infected with TotoLuc1101 and treated with 0.1 mM DTT from 0 hpi. At the times indicated, luciferase activity and the phosphorylation status of eIF2α were measured in extracts.