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. 2006 Jan 1;20(1):113–124. doi: 10.1101/gad.366606

Figure 5.

Figure 5.

oriC and terC localization after inactivation of RNAP. Simultaneous nucleoid and oriC or nucleoid and terC labeling. Cells of MC1000 rpoC907 oriC::parS at 30°C (A), MC1000 rpoC907 oriC::parS at 39°C (B), MC1000 rpoC907 terC::parS at 30°C (C), and MC1000 rpoC907 terC::parS at 30°C (D) were grown in AB minimal medium at 30°C for eight generations and then shifted to 39°C for two generations. Cells in the right panel were treated with cephalexin for two mass doublings; the drug was added simultaneously with the shift to nonpermissive temperature. oriC and terC were visualized by GFP-ParB (encoded by pTK536 also present in the cells) binding to parS sites inserted in bglF (near oriC) or in relBE (near terC). DNA was visualized by DAPI staining.