Figure 3.

Expression of the DN-WT1 transgene. (A) The DN-WT1 transgene. Solid bar is DN-WT1 coding region; see Figure 1 for definition of abbreviations. (B) RNase protection analysis of adult testes RNA (20 μg) from the DN1 and DN7 lines, performed with a WT1 probe that distinguishes between endogenous and transgene transcripts. Transgene transcripts were expressed at ∼1.2-fold and approximately twofold higher levels than endogenous WT1 transcripts in the testes of DN1 and DN7 mice, respectively (when corrected for [³²P]UTP content in the protected bands). (C) RNase protection analysis of tissue RNA (20 μg) from an adult DN7 mouse annealed with the transgene-specific probe used in Figure 1C.