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. 2006 Jan 15;20(2):159–173. doi: 10.1101/gad.1392506

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Figure 2. — Lineage analyses of unstable ChrVIIs in genetically unstable cells. (A) The logic and methodology of the lineage analysis. Two cells (hatched and open) were plated on the selection plate containing canavanine and not containing adenine. The two cells generate a round and a sectored colony, respectively. The phenotypes of individual cells in each colony were determined as shown (see text and Materials and Methods). Because the hatched founder cell was genetically stable, it generated a round colony, and consequently colonies on the analytical plate of one phenotype. Because the open founder cell was unstable, it generated a sectored colony, and consequently colonies on the analytical plate of many different phenotypes (open, gray, black). Note that some colonies on the analytical plates contain cells with heterogeneous phenotypes. If the founder cell on the analytical plate undergoes a genetic change in the first or second cell division, the colony will contain cells of two different phenotypes in two halves of the colony (or in one-fourth and three-fourths of the colony), detectable as a “fragmented” colony (see text). (B) Phenotypes of colonies on analytical plates. The phenotypes of cells from selected colonies that gave rise to a colony on an analytical plate. Phenotypes were determined by selective replica plating. For example, all 132 cells from round colony #1 gave rise to analytical colonies with an E2 phenotype, while 51 cells from colony #4 gave rise to colonies of six different phenotypes. A total of 443 cells from nine sectored rad9 colonies were analyzed, and the absolute numbers and percent of each colony phenotype are shown. Percentages were rounded to the nearest integer. See text for more details. (C) Extended lineage analysis of unstable recombinant ChrVIIs in unstable E2 cells. In the lineage analysis to the left, an E2 cell called E2-1 gave rise to cells in which 75% had lost the chromosome and 25% had retained the chromosome. Three of those E2 cells were subjected to lineage analysis, and the results are shown. Analysis of a second E2 cell is shown on the right. See text for discussion. (D) Summary of the fates of an unstable ChrVII in a genetically unstable cell. An initial cell with two ChrVII homologs undergoes chromosome changes by multiple undefined steps to form a cell with an unstable ChrVII that has a chromosomal translocation (dasemonstrated in subsequent sections). An unstable E2 ChrVII is shown. A cell with an unstable ChrVII generate cells with further ChrVII rearrangements or loss as shown. See text for discussion.

Figure 2. — Lineage analyses of unstable ChrVIIs in genetically unstable cells. (A) The logic and methodology of the lineage analysis. Two cells (hatched and open) were plated on the selection plate containing canavanine and not containing adenine. The two cells generate a round and a sectored colony, respectively. The phenotypes of individual cells in each colony were determined as shown (see text and Materials and Methods). Because the hatched founder cell was genetically stable, it generated a round colony, and consequently colonies on the analytical plate of one phenotype. Because the open founder cell was unstable, it generated a sectored colony, and consequently colonies on the analytical plate of many different phenotypes (open, gray, black). Note that some colonies on the analytical plates contain cells with heterogeneous phenotypes. If the founder cell on the analytical plate undergoes a genetic change in the first or second cell division, the colony will contain cells of two different phenotypes in two halves of the colony (or in one-fourth and three-fourths of the colony), detectable as a “fragmented” colony (see text). (B) Phenotypes of colonies on analytical plates. The phenotypes of cells from selected colonies that gave rise to a colony on an analytical plate. Phenotypes were determined by selective replica plating. For example, all 132 cells from round colony #1 gave rise to analytical colonies with an E2 phenotype, while 51 cells from colony #4 gave rise to colonies of six different phenotypes. A total of 443 cells from nine sectored rad9 colonies were analyzed, and the absolute numbers and percent of each colony phenotype are shown. Percentages were rounded to the nearest integer. See text for more details. (C) Extended lineage analysis of unstable recombinant ChrVIIs in unstable E2 cells. In the lineage analysis to the left, an E2 cell called E2-1 gave rise to cells in which 75% had lost the chromosome and 25% had retained the chromosome. Three of those E2 cells were subjected to lineage analysis, and the results are shown. Analysis of a second E2 cell is shown on the right. See text for discussion. (D) Summary of the fates of an unstable ChrVII in a genetically unstable cell. An initial cell with two ChrVII homologs undergoes chromosome changes by multiple undefined steps to form a cell with an unstable ChrVII that has a chromosomal translocation (dasemonstrated in subsequent sections). An unstable E2 ChrVII is shown. A cell with an unstable ChrVII generate cells with further ChrVII rearrangements or loss as shown. See text for discussion.