Table 2.

Summary of kinetic data

FGF		FGFR1c	FGFR2c	FGFR3c	FGFR4	FGFR2b	FGFR3b
FGF8a	kon (/M/s)a	1.24 × 104	1.67 × 104	4.22 × 103	1.44 × 104	NBc	NB
	koff (/s)a	1.55 × 10−2	1.81 × 10−2	1.08 × 10−2	1.62 × 10−2	NB	NB
	KD (M)b	1.25 × 10−6	1.08 × 10−6	2.45 × 10−6	1.13 × 10−6	NB	NB
FGF8b	kon (/M/s)	9.35 × 103	3.76 × 104	2.78 × 104	4.77 × 104	NB	NB
	koff (/s)	1.18 × 10−3	5.84 × 10−3	3.77 × 10−3	0.0235	NB	NB
	KD (M)	1.26 × 10−7	1.55 × 10−7	1.36 × 10−7	4.92 × 10−7	NB	NB
FGF17	kon (/M/s)	1.77 × 104	7.34 × 104	8.02 × 103	2.25 × 104	NB	NB
	koff (/s)	6.8 × 10−3	3.2 × 10−2	5.5 × 10−3	2.74 × 10−2	NB	NB
	KD (M)	3.82 × 10−7	4.32 × 10−7	6.87 × 10−7	1.22 × 10−6	NB	NB
FGF18	kon (/M/s)	1.39 × 104	6.07 × 104	1.16 × 104	4.45 × 104	NB	NB
	koff (/s)	4.0 × 10−3	2.5 × 10−2	8.0 × 10−3	4.0 × 10−2	NB	NB
	KD (M)	2.84 × 10−7	4.11 × 10−7	7.02 × 10−7	9.1 × 10−7	NB	NB
FGF8bF32A	kon (/M/s)	8.65 × 103	8.93 × 103	3.93 × 103	6.49 × 103	NB	NB
	koff (/s)	9.61 × 10−3	9.29 × 10−3	5.14 × 10−3	2.37 × 10−2	NB	NB
	KD (M)	1.11 × 10−6	1.04 × 10−6	1.31 × 10−6	3.65 × 10−6	NB	NB
FGF8bCTR	kon (/M/s)	9.66 × 103	4.13 × 104	2.08 × 104	2.41 × 104	NB	NB
	koff (/s)	1.0 × 10−3	4.2 × 10−3	2.4 × 10−3	1.5 × 10−2	NB	NB
	KD (M)	1.05 × 10−7	1.03 × 10−7	1.14 × 10−7	6.12 × 10−7	NB	NB
FGF8bNTR	kon (/M/s)	2.47 × 104	3.49 × 104	2.48 × 104	4.05 × 104	NB	NB
	koff (/s)	1.87 × 10−3	6.88 × 10−3	3.81 × 10−3	2.27 × 10−2	NB	NB
	KD (M)	7.57 × 10−8	1.97 × 10−7	1.54 × 10−7	5.6 × 10−7	NB	NB

^ak_on and k_off were derived as described in Materials and Methods. ϰ² was <10% in all cases.

^bThe apparent affinity, K_D is equal to k_off/k_on.

^c(NB) Negligible binding.