Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of libraries that are enriched for SINEC_Cf elements and flanking sequence. (A) Genomic DNA is cleaved with the frequently cutting restriction enzyme, NlaIII. (B) The cleaved fragments are self-ligated. (C) The circularized products are subjected to PCR using SINEC_Cf-specific primers. (D) The linear products are size-selected and cloned in a plasmid vector. (E) Inserts are sequenced with a vector-specific primer.
