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. 2006 Jan;16(1):1–10. doi: 10.1101/gr.4222606

Figure 3.

Figure 3.

Reporter activity of promoter deletion constructs. (A) Diagram of promoter deletion constructs. (B) Average promoter activity observed for each of the 6 constructs of decreasing upstream sequence (1000 bp, 500 bp, 350 bp, 200 bp, 90 bp, 40 bp). The average represents normalized activity of constructs in 45 promoters and seven cell lines (HT1080, HeLa, HCT116, G-402, AGS, T98G, and JEG3). The promoter activity, assayed in triplicate and represented as normalized firefly luciferase/Renilla luciferase ratio, provides a transfection-normalized value to compare activity within and between cell lines. (C) Average activities of promoter fragments for the UDP glycosyltransferase gene (UGT1A10) across seven cell types. (D) Average activities of sperm-associated antigen 4 (SPAG4) promoter fragments across seven cell types. The 898-bp fragment of the SPAG4 promoter shows considerably less activity than the 372-bp fragment.