Figure 3.

Northern blot analysis of the size and tissue distribution of selected ncRNAs. Details of the methods can be found in the Supplemental material. The full-length cDNA probes of the eight ncRNAs used in this analysis were obtained from the DNABook (Hayashizaki and Kawai 2004). The corresponding GenBank IDs (and sizes) are: (A) AK035433 (1318 bp); (B) AK028310 (2905 bp); (C) AK017092 (765 bp); (D) AK040014 (139 bp); (E) AK040058 (1141 bp); (F) AK014924 (1799 bp). Total RNA was used, and blots were exposed in a PhosphorImager cassette for 24 h at 4°C. The positions and sizes (in kilobases) of molecular mass markers are shown.