Figure 2.

The three computationally predicted chimeras in the ENCODE regions verified by RT-PCR. (A) chimera ENm013_GCL+006; (B) chimera ENr331_GCL+002; (C) chimera ENm005_GCL-002. Only one had known chimeric mRNAs before our experiments. One of the chimeras—ENm005_GCL-002—still lacks known mRNAs. The results of the RT-PCR validation in 12 of the 24 tissues tested are shown. Asterisks mark positive amplimers. The tissues are (1) brain, (2) heart, (3) kidney, (4) spleen, (5) liver, (6) colon, (7) small intestine, (8) muscle, (9) lung, (10) stomach, (11) testis, and (12) placenta. Chimeras were tested in 12 additional tissues (not shown here).