Figure 2.

Bovine-human comparative map. (A) Twenty-two BAC clones constitute the minimum tiling path between BMS2790 and INRA003. Genes and map positions in HSA1p21 are based on the human genome draft sequence. Dotted lines indicate the gene content of the BAC clones. The positions (in cM) of microsatellite markers ILSTS029, BMS2790, and INRA003 on the bovine linkage map are indicated. (B) Differential proteome patterns of cardiac and skeletal tissues from CVM affected and unaffected (wt) calves. The four images display segments from silver-stained high-resolution 2DE maps. The encircled areas show the characteristic electrophoretic pattern of α1-antitrypsin that was observed only in tissues from affected CVM calves. The mobility changes with a shift in pI as well as in MW indicate that aberrant protein glycosylation is associated with CVM. Moreover, the increased spot intensity of α1-antitrypsin in disease-affected tissue relative to normal tissue suggests that the abnormally glycosylated variants accumulate in the tissues of affected calves. The pH spans are indicated along the x-axis and the approximate molecular weight is on the y-axis.