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. 2002 Sep;22(18):6582–6591. doi: 10.1128/MCB.22.18.6582-6591.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — GTP/GDP loading of Raichu-Rac and Raichu-Cdc42. 293T cells expressing Raichu-Rac, Raichu-Cdc42, Flag-tagged Rac, and Flag-tagged Cdc42 were labeled with ³²P_i. The G proteins were precipitated with either anti-GFP rabbit serum or an anti-Flag monoclonal antibody, followed by TLC analysis. GTP and GDP were quantitated with a BAS-1000 image analyzer, and values of GTP/(GTP + GDP) with standard deviations were plotted. Asterisks indicate that no ³²P_i-labeled guanine nucleotides were detected.