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. 2002 Sep;22(18):6582–6591. doi: 10.1128/MCB.22.18.6582-6591.2002

FIG. 3.

FIG. 3.

Imaging of Rac and Cdc42 activities in HT1080 cells. (A) HT1080 cells were transfected with expression vectors as indicated on the left. After 48 h, cells expressing EGFP-Rac or EGFP-Cdc42 were photographed for EGFP and phase contrast (PC) images. Cells expressing Raichu-Rac or Raichu-Cdc42 were imaged for YFP, CFP, and differential interference contrast (DIC). In the IMD mode images (Ratio), eight colors from red to blue are used to represent the YFP/CFP ratio, with the intensity of each color indicating the mean intensity of YFP and CFP. The upper and lower limits of the ratio range are shown on the right. (B) Increase in the CFP emission by YFP photobleaching. HT1080 cells expressing Raichu-Rac or Raichu-Cdc42 were photobleached at an excitation wavelength of 510 nm for 10 min. Emission intensities of CFP and YFP in this cell were monitored every 30 s and are shown at the left. Cell images of pre- and postbleaching are shown at the right.