FIG. 6.

Activity of Rac and Cdc42 video imaged with Raichu-CRIB-X. (A) Increase in the CFP emission by YFP photobleaching. HT1080 cells expressing Raichu-CRIB-X were photobleached at an excitation wavelength of 510 nm for 10 min. Emission intensities of CFP and YFP in these cells (left) were monitored every 30 s. Cell images of pre- and postbleaching are also shown (right). (B) HT1080 cells transfected with pRaichu-CRIB-X were replated onto a collagen-coated glass base dish. Beginning 1 h after replating, CFP, YFP, and differential interference contrast (DIC) images were obtained every 2 min with a time-lapse microscope as described in the legend to Fig. 4. Arrows point in the direction of cell migration. The upper and lower limits of the ratio range are shown on the right. The original video images are presented on our website (:http://www-tv/biken.osaka-u.ac.jp/rei/).