FIGURE 2. Western blot analysis for IκB-α (A) and RelA (B) protein expressions were performed on total and nuclear cell extracts (50 μg protein/lane), respectively, from both esophageal tumor tissues and non tumor tissues and separated by 10% acrylamide SDS-PAGE, blotted onto PVDF membrane and probed with specific antisera. Experiments were performed 3 times and results of 6 independent patients are shown.