Figure 6.

The Leu262Pro mutation in the gene encoding the γ subunit of the F₁ moiety from a Dk strain of T. brucei converts the petite-negative yeast K. lactis into a petite-positive form. The Leu to Pro mutation was introduced into the conserved residue of the ATP synthase γ subunit of K. lactis (Leu265 in ATP3) and the mutant allele (atp3-3) expressed in a strain lacking the endogenous ATP3 gene. Control strains expressed the wild-type ATP3 gene or a suppressor mutation identified previously (atp3-2). Growth was analyzed after serial dilution on plates containing the carbon sources glucose (Glu), glycerol (Gly), or glucose medium with 16 μg/ml EB. Plates were incubated at 28°C for 2, 6, and 9 days, respectively.