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. 2005 Nov 10;24(23):4018–4028. doi: 10.1038/sj.emboj.7600863

Figure 4.

Figure 4

Effects of SIKE on assembly of the TRIF-IKKɛ-IRF-3, TRIF-TBK1-IRF3 and TRIF-TRAF6-RIP complexes. (A) SIKE disrupts the TRIF-IKKɛ-IRF-3 complex. The 293 cells (1 × 106) were transfected with 5 μg of the indicated plasmids. Cell lysates were immunoprecipitated with control mouse IgG (IgG) or anti-Flag (αFlag) antibody. The immunoprecipitates were analyzed by Western blot with anti-HA antibody (upper panel). The expression levels of the transfected proteins were detected by Western blot analysis with anti-HA and anti-Flag antibodies (low panel). (B) SIKE disrupts the TRIF-TBK1-IRF-3 complex. The experiments were carried out similarly as in (A), except that TBK1 plasmid was used to replace IKKɛ plasmid. (C) SIKE does not disrupt the interactions of TRIF with TRAF6 or RIP. The 293 cells were transfected with the indicated plasmids and the co-immunoprecipitation and Western blot analysis were carried out as in (A).