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. 2005 Nov 3;24(23):4041–4051. doi: 10.1038/sj.emboj.7600864

Figure 2.

Figure 2

Identification of a knockout mutant carrying a T-DNA insertion in AtNRAMP4 gene. (A) Localization of the T-DNA insertions in nramp3-1 and nramp4-1 mutant alleles. (B) AtNRAMP4 protein is not detectable in nramp4-1 homozygous mutant line. Western blot showing abundance of AtNRAMP4 protein in roots and shoots of Fe-starved or -replete A. thaliana wild type or nramp4-1 homozygous seedlings. 20 μg of proteins was loaded in each lane. Growth conditions as in Figure 1E and F. Note that anti-AtNRAMP4 antibodies recognize two additional proteins that are not affected in nramp4-1 mutants. (C) Seedling development is not affected differentially in wild type, nramp3-1 and nramp4-1 grown under a range of Fe supply. Mean root length±s.e. (n=15–25) of wild type (black bars), nramp3-1 (light gray bars) or nramp4-1 mutant (dark gray bars) measured after 16 days of growth on ABIS media containing 0.1–100 μM FeHBED (as indicated) as the sole source of Fe.