Skip to main content
. 2005 Nov 17;24(23):4052–4060. doi: 10.1038/sj.emboj.7600878

Figure 1.

Figure 1

PIR+ cells in the IL-7R+ population of FL are T/NK/DC lineage restricted. (A) Emergence of PIR+ cells in the Lin−c-kit+IL-7R+ population during FL ontogeny. FL cells of various gestational days from Balb/c mice were four-color stained with anti-Lin, anti-c-kit, anti-PIR, and anti-IL-7R. Numbers in the left and right panels represent the percentage of cells gated in each box in whole FL cells, and that in the Lin−c-kit+ fraction, respectively. (B) Single cells of various populations from 13 dpc FL were individually examined by the MLP assay that covers T, B and myeloid lineages. The bars represent the numbers of progenitors detected among the 100 cells analyzed. (C) A profile of cells generated from a single 13 dpc PIR− FL progenitor on day 12 in simple FTOC conditions. (D) Profiles of cells generated on day 12 from a single 13 dpc PIR+ FL progenitor in FTOC conditions. (E) A total of 50 PIR+ FL cells from 13 dpc fetuses of EGFP Tg mice were individually analyzed for T, NK and DC potential by the MLP assay that covers T, NK and DC lineages. p-T/NK represent progenitors generating T and NK cells. The green portion of the columns indicates the number of progenitors that showed DC generation. (F) A profile of cells generated from a single PIR+ FL progenitor in the MLP assay conditions for T, NK and DC lineages. (G) A photomicrograph of EGFP+ DC generated in FTOC from a single PIR+ progenitor. Bar indicates 50 μm.