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. 2005 Nov 17;24(23):4052–4060. doi: 10.1038/sj.emboj.7600878

Figure 3.

Figure 3

Expression of PIR visualizes the thymic immigration pathway. (A) FB cells at 11 and 13 dpc were four-color stained in the same manner as in Figure 1A. Profiles of cells gated on Lin−c-kit+ fraction (1.2 and 0.5% of whole FB cells at 11 and 13 dpc, respectively) are shown. The numbers in the panels represent the percentage of cells gated in each box in Lin−c-kit+ FB cells. (B) Tissue containing the 11 dpc thymic anlage and surrounding mesenchyme was taken, digested and stained in four colors with anti-CD45, anti-c-kit, anti-PIR and anti-IL-7R. (C) A frozen section of an 11 dpc fetus containing the thymic anlage region was stained in two colors with an anti-PIR antibody (green) and an anti-Keratin antibody (red), and a serial section was stained with the anti-PIR antibody (green) and an anti-IKAROS antibody (red).