FIG. 6.

Sp1 interacts with the BAF complex in vivo. (A) Association of Sp1 with the IFITM3 promoter does not require the activity of the BAF complex. The immunoprecipitated chromatin from SW-13 cells with each antibody was analyzed, respectively, with the IFITM3 promoter primers and the negative control primers by real-time PCR. The graph shows the relative enrichment of the IFITM3 promoter sequence after normalization to the negative control sequence. The error bar indicates the range from two experiments. (B) Sp1 interacts with the BAF complex in vivo. Nuclear extracts prepared from DSP-cross-linked cells were immunoprecipitated with the indicated antibodies. The immunoprecipitates were resolved by SDS-PAGE and blotted with BRG1 or Sp1 antibodies. One percent of the nuclear extracts used for IP was loaded in lanes 1 and 5 as input. The size of the band is indicated on the left of the panels.