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. 2005 Nov 17;24(23):4188–4197. doi: 10.1038/sj.emboj.7600881

Figure 3.

Figure 3

Dependence of the translocation activity on the free MTase in solution. The three time traces belong to consecutive experiments on the same DNA molecule. At t0=0 s, motor activity was initiated by adding 20 nM MTase and 20 nM HsdR into the flow cell. At t0=600 s, the free MTase in solution is removed by adding 250 μl (∼3-fold flow cell volume) of 20 nM HsdR only. After t0=1200 s, 250 μl of 100 nM HsdR is added. Translocation activity continued for another 1800 s and was terminated by the loss of the DNA molecule.