Table 1.
Expression of full-length HK853 in E. coli
| T (°C) | Distribution of HK853 between cellular compartments (%) |
HK853 portion of total protein within each cellular compartment (%) |
||
|---|---|---|---|---|
| Membrane | Soluble | Membrane | Soluble | |
| 20 | 5.0±0.8 | 95.0±0.8 | 31.5±16.1 | 32.0±6.4 |
| 25 | 8.5±1.2 | 91.5±1.2 | 57.7±6.5 | 33.0±4.5 |
| 30 | 14.6±2.7 | 85.4±2.7 | 60.9±5.5 | 26.3±3.8 |
| 37 |
25.9±7.7 |
74.1±7.7 |
70.4±5.9 |
26.1±10.3 |
| Results are averages of two independent experiments, each of which was quantified twice (two independent gels). Possible inclusion bodies were eliminated before analysis. | ||||