Table 1.

Summary of the apparent collagen binding affinities of the different recombinant fragments of CNA

Construct	KDapp (μM)a
CNA31–531	2.2±0.4
CNA31–344	0.2±0.02
CNA31–318	1.5±0.5
CNA151–318	31.6±4.4
CNA141–344	69.6±6.1
CNA151–531	28.3±3.3
CNA141–531	38.1±11.8
CNA31–156	NDb
CNA319–531	NDb
aThe apparent dissociation constants (KDapp) were determined from ELISA assays as described in Materials and methods. Recombinant fragments of the different constructs were added to wells coated with bovine type I collagen. Bound proteins were detected with mouse anti-His antibodies and goat-anti-mouse IgG-AP conjugants. Data analysis was performed using the nonlinear regression method (GraphPad Prism).
bCNA31–156 and CNA319–531 did not show any binding in the ELISA assays and their KDapp values were not determined.