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. 2002 Sep;22(18):6648–6660. doi: 10.1128/MCB.22.18.6648-6660.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 9. — Drosophila and human SLBPs are not interchangeable. (A) Diagram of dSLBP and human SLBP (hSLBP) and a hybrid protein H-D-H composed of the RBD from dSLBP (dRBD) and both flanking domains from human SLBP (hN and hC). (B) The mouse H1t (left panel) and dH3∗ (right panel) pre-mRNAs were processed in the species-specific SLBP-depleted nuclear extracts alone (lane 2 in each panel) or in the presence of the baculovirus-expressed proteins, as indicated. Lane 1 in each panel shows processing in the control, undepleted nuclear extract. (C) Drosophila and human SLBPs inhibit processing in the heterologous nuclear extract. The Drosophila dH3∗ (left panel) and mouse H2a (right panel) pre-mRNAs were incubated in the Drosophila and mouse nuclear extracts, respectively (lane 1 in each panel) and in the same extracts in the presence of the indicated baculovirus-expressed SLBPs (lanes 2 and 3 in each panel).