EMSA experiments |
aadA7-TB1 |
GATCCTGCCTAACAATTCATTCA |
aadA7-TB2 |
TGCAGCAATTGCCTAACGCTTG |
aadA7bot |
TGCAGCAATTGCCTAACGCTTGAATTAAGCCGCGC CGCGAAGCGGCGTCGGCTTGAATGAATTGTTAGGC AGGATC |
aadA7top |
GATCCTGCCTAACAATTCATTCAAGCCGACGCCGC TTCGCGGCGCGGCTTAATTCAAGCGTTAGGCAATT GCTGCA |
attI1-TB1 |
GCGGGATCCGCACTAACTTTG |
attI1-TB2 |
GGAATTCAGCAGCAACGATG |
attI1bot |
GCGGGATCCGCACTAACTTTGTTTTAGGGCGACTG CCCTGCTGCGTAACATCGTTGCTGCTGAATTCCGG |
attI1top |
CCGGAATTCAGCAGCAACGATGTTACGCAGCAGGG CAGTCGCCCTAAAACAAAGTTAGTGCGGATCCCGC |
VCR-TB1 |
GCGGGATCCGTTATAACGCC |
VCR-TB2 |
CCGGAATTCGTTATAACAAACGC |
VCRbot |
GCGGGATCCGTTATAACGCCCGCCTAAGGGGCTGA CAACGCACTACCACTAAACTCAAACACAACAACAG CAACCAC |
|
CGCGGCTCAATGGGACTGGAAACGCCACGCGTTGA CAGTCCCTCTTGAGGCGTTTGTTATAACGAATTGG |
VCRtop |
CCGGAATTCGTTATAACAAACGCCTCAAGAGGGAC TGTCAACGCGTGGCGTTTCCAGTCCCATTGAGCCG CGGTGGT |
|
TGCTGTTGTTGTGTTTGAGTTTAGTGGTAGTGCGT TGTCAGCCCCTTAGGCGGGCGTTATAACGGATCGC |
|
Plasmid constructions |
Fmal2 |
CCGGAATTCTAATAGGAGACCCGGGATGAAAACCG CCACTGCGCC |
Eibam2 |
CGCGGATCCTTACCTCTCACTAGTGAGGGGC |
Isal/sac1 |
CGCGGATCCCATATGCTCGAGGAGCTCGCCGGCCA GCCTCGCAGAGCAGGATTCCCG |
Isal/sac2 |
CGCGGATCCGCTAGCGAATTCGTCGACCAGCTTTT GTTCCCTTTAGTGAGGGTTAATTGCG |
Ikpn/sal-1 |
TCCCCCGGGCATATGCTCGAGGGTACCGGTATCGA TAAGCTTGATATCGAATTCAGATCTG |
Ikpn/sal-2 |
TCCCCCGGGGCTAGCGAATTCGTCGACCAATTCGC CCTATAGTGAGTCGTATTACGCGCGC |
Ikpn/sac-1 |
TCCCCCGGGCATATGCTCGAGGGTACCCACCGCGG TGGCGGCCGCTCTAGAACTAGTGGAT |
Ikpn/sac-2 |
TCCCCCGGGGCTAGCGAATTCGAGCTCCAATTCGC CCTATAGTGAGTCGTATTACGCGCGC |
Isal/sac-3 |
CGGGGTACCCATATGCTCGAGGAGCTCCCGCTCTA GAACTAGTGGATCCCCCGGGCTGCAG |
Isal/sac-4 |
CGGGGTACCGCTAGCGAATTCGTCGACCAGCTTTT GTTCCCTTTAGTGAGGGTTAATTGCG |
Imfe/sac-1 |
CGGGGTACCCATATGCTCGAGCAATTGCACCGCGG TGGCGGCCGCTCTAGAACTAGTGGAT |
Imfe/sac-2 |
CGGGGTACCGCTAGCGAATTCGAGCTCCGCCGAAT AAATACCTGTGACGGAAGATCACTTC |
attB-1 |
AATTCAGCCTGCTTTTTTATACTAACTTGG |
attB-2 |
GATCCCAAGTTAGTATAAAAAAGCAGGCTG |
attC-GD1 |
GATCCTGCCTAACAATTCATTCAAGCCGACGCCGC TTCGCGGCGCGGCTTAATTCAAGCGTTAGGCAATT GCTGCA |
attC-GD2 |
GCAATTGCCTAACGCTTGAATTAAGCCGCGCCGCG AAGCGGCGTCGGCTTGAATGAATTGTTAGGCAG |
attC-GD3 |
GATCCTGCCTAACAATTCATTCAAGCCGACGCCGC TTCGCGGCGCGGCTTAATTCAAGCGTTAGGC |
attC-GD4 |
AATTGCCTAACGCTTGAATTAAGCCGCGCCGCGAA GCGGCGTCGGCTTGAATGAATTGTTAGGCAG |
attC-Mut1 UP |
CTAGAATTCGGTTATAACAATTCATTCAAGCCGAC CATAGTTCGCGGCGC |
attC-Mut1 DW |
CGCGGATCCGGTTATAACGCTTGAATTAAGCCGCG CCGCGAACTATGGTC |
attC-Mut2 UP |
CTAGAATTCGGTTATAACAATTCATTCAAGCCGAC CATAGTTCCTATGGCGGC |
attC-Mut2 DW |
CGCGGATCCGGTTATAACGCTTGAATTAAGCCGCC ATAGGAACTATGGTCGGC |
attC-Mut3 |
CTAGAATTCGGTTATAACAATTCATTCAACGGGAC CATAGTTCGCGGCGC |
attC-Mut4 UP |
CTAGAATTCGGTTATAACAATTCATTCAACGGGAC CATAGTTCCTATGGCCCG |
attC-Mut4 DW |
CGCGGATCCGGTTATAACGCTTGAATTAACGGGCC ATAGGAACTATGGTCCCG |
MRV |
AGCGGATAACAATTTCACACAGGA |
SW23begin |
CCGTCACAGGTATTTATTCGGCG |
SW23end |
CCTCACTAAAGGGAACAAAAGCTG |