FIG. 2.

Identification of the IRP2-binding domain in YB-1. (A) Schematic illustration of the full-length and deleted GST-YB-1 proteins used in this study. The striped box indicates the cold shock domain. (B) GST-pull down assay. 1 μg of each GST-YB-1 deletion mutant and GST which binds to 30 μl of glutathione-Sepharose 4B beads were incubated with a 100-fold excess of full-length Thio-IRP2 in the presence or absence of 50 μM FAC. Proteins bound to the beads were resolved by SDS-PAGE and analyzed by Western blotting using an anti-Thio antibody.