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. 2005 Dec 1;25(1):139–149. doi: 10.1038/sj.emboj.7600900

Figure 3.

Figure 3

HDAC1 recruitment and loss from the latent HIV LTR RNA Pol II complexes. (A) HDAC1 is present on the latent HIV-1 promoter and is lost when T cells are activated. Fixed chromatin extracts from J-Lat 6.3 cells treated with 20 ng/ml TNF-α for 30 min or left untreated were immunoprecipitated with the indicated antibodies. Samples were analyzed for enrichment in HIV LTR DNA by UV visualization of PCR products on an ethidium bromide-stained agarose gel. Specific enrichment was quantitated by real-time PCR; mean of three measurements is indicated beneath each band image. (B) HDAC1 is excluded from RelA- or RNA Pol II-containing complexes on the HIV-1 LTR. Fixed chromatin extracts from J-Lat 6.3 cells treated as in panel A were immunoprecipitated with the indicated antibodies and enriched complexes were subjected to a second round of immunoprecipitation with the indicated antibodies. Data are representative of three independent experiments.