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. 2005 Dec 1;25(1):139–149. doi: 10.1038/sj.emboj.7600900

Figure 6.

Figure 6

p50 and TSA-sensitive HDACs mediate desensitization of the latent HIV LTR to Tat. (A) TSA enhances Tat induction of latent HIV expression. J-Lat cells were transfected with control, Tat, or RelA expression vectors, pulse treated with TSA (400 nM) for 1 h or left untreated, and transfected cells were assessed for GFP expression (left panel). Lysates were prepared and probed for β-actin, RelA, and Tat expression as a control (right panel). Note the low basal sensitivity of J-Lat cells to Tat induction and the sensitization to Tat induced by TSA treatment. (B) TSA treatment does not alter inherent Tat transactivating potential. Jurkat cells were transfected with an HIV LTR firefly luciferase reporter vector and a control Renilla luciferase vector in conjunction with control, Tat, or RelA expression vectors, pulse treated with TSA (400 nM) for 1 h or left untreated, and relative increase in firefly luciferase activity was quantitated. (C) p50 shRNA enhances Tat induction of latent HIV expression. Scramble- or p50-shRNA stable cells were transfected with control, Tat, or RelA expression vectors, pulse treated with TSA (400 nM) for 1 h or left untreated, and transfected cells were assessed for GFP expression (left panel). Lysates were prepared and probed for β-actin, RelA, and Tat expression as a control (right panel). Note the sensitization to Tat expression in p50-shRNA cells and relative lack of additional TSA sensitivity.