Table 2:

Calculated hemolysis index (HI), mg/dL hemoglobin, required to increase ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) concentration by 10% and to reach 400 pg/mL, based on quadratic models.

Patient	Baseline UCH-L1 concentration (pg/mL)	Baseline HI	UCH-L1 concentration increased by 10%	HI required for 10% UCH-L1 increase	HI required to reach 400 pg/mL of UCH-L1	Quadratic equation
1	44	3	48	17	2106	43,5 + 0,259x - 4,26E-05x2 =y
2*	58	0	64	13	856	58.7 + 0.405x - 7.17E–06x2 =y
3*	65	11	71	40	2275	64,6 + 0,161x - 5,97E-06x2 =y
4	68	1	74	13	754	66.9 + 0.549x - 1.42E–04x2 =y
5	81	2	89	42	1795	78,3 + 0,257x - 7,16E-05x2 =y
6*	96	1	106	39	1929	95,5 + 0,272x - 7,05E-05x2 =y
7	106	1	116	26	723	105 + 0.441x - 4.59E–05x2 =y
8	122	4	134	24	559	122 + 0,502x - 7,68E-06x2 =y
9*	134	1	148	194	2103	134 + 0.0668x + 2.84E–05x2 =y
10*	136	1	149	73	1484	139 + 0.134x + 2.82E–05x2 =y
11	140	3	154	25	369	134 + 0.807x - 2.33E-04x2 =y
12	142	10	156	42	775	139 + 0,41x - 9,47E-05x2 =y
13	158	5	174	84	1310	156 + 0,216x - 2,27E-05x2 =y
14	169	59	186	102	1009	153 + 0,341x - 1,69E-04x2=y
15	178	2	196	33	573	177 + 0.583x - 3.38E–04x2=y
16	232	110	255	171	468	171 + 0,492x - 5,63E-06x2=y
17	247	38	272	195	968	241 + 0.158x + 6.44E–06 x2=y
18	262	73	288	138	269	154 + 1,03x + -4,29E-04x2=y
19	413	54	455	95	N/A	350 + 1,13x + -2,02E-04x2=y
20	1183	164	1301	808	N/A	1206 + -0,232x + 4,33E-04x2=y

The equations were derived from individually fitted quadratic models describing the relationship between the HI and the measured UCH-L1 concentration in samples with progressively increasing hemolysis. The patients’ results are shown in order of increasing baseline UCH-L1 concentration.

*Serum samples (*); remaining samples are lithium heparin plasma.

N/A: not appicable